期刊
FOOD CONTROL
卷 31, 期 1, 页码 65-70出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2012.09.006
关键词
Zearalenone; HTS-ELISA; Corn; China
资金
- Qiqihar University [000240]
- Federal Target Program Scientific and scientific-pedagogical personnel of innovative Russia [16.740.11.0158]
- Russian Foundation for Basic Research
High-throughput screening enzyme-linked immunosorbent assay (HTS-ELISA) method was used for screening of hybridoma cells that secret high-affinity anti-zearalenone (ZON) monoclonal antibody (mAb). Recovery rate of ZON from spiked corn samples was between 80% and 128%. The cross-reactivities of the anti-ZON mAb against ZON, beta-zearalenol, alpha-zearalanol, beta-zearalanol, deoxynivalenol and aflatoxin B1 were 100%, 24.1%, 189.1%, 43.9%, <1.0%, <1.0%, respectively. The limit of detection for ZON was 0.1 ng/g. The linear range for the developed indirect competitive ELISA was 0.5-10 ng/g. The half maximal,(50%) inhibitory concentration (IC50) of the anti-ZON mAb was 1.79 ng/g. In total, 137 samples of corn, powdered corn, and processed corn products were collected from farms and supermarkets in northeastern China. Of 42 corn samples tested, 4 (9.5%) samples contained ZON 5-60 ng/g, 4 (9.5%) samples contained 60-500 ng/g, In 34(81%) samples ZON was not detected. Of 15 powered corn samples, only 1 (6.7%) sample contained 5-60 ng/g, and no ZON was detected in 80 processed corn products. (C) 2012 Elsevier Ltd. All rights reserved.
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