期刊
FOOD CONTROL
卷 25, 期 1, 页码 190-196出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2011.10.005
关键词
Pediocin PA-1/AcH; Nham; Listeria monocytogenes; Application
资金
- Japan Society for the Promotion of Science (JSPS)
- National Research Council of Thailand (NRCT)
- National Science and Technology Development Agency of Thailand (NSTDA)
Lactic acid bacteria forming bacteriocin active against Listeria monocytogenes were screened for potential use in controlling growth of L. monocytogenes in Nham, a Thai traditional fermented pork sausage. Based on the spot-on-lawn assay, Pediococcus pentosaceus BCC 3772 was found to produce the highest anti-listens activity. Through a series of chromatographic techniques, Edman N-terminal sequencing and mass spectrometry, this anti-listeria compound showed a complete homology to pediocin PA-1/AcH. Inoculation of P. pentosaceus BCC 3772 in Nham caused a significant decrease of 3.2 log in population of spiked L monocytogenes within 18-24 h of fermentation, in comparison with the initial count with no significant changes in sensory properties and consumer acceptability on the overall characteristics of the final fermented Nham products. These results indicate that the P. pentosaceus BCC 3772 should be useful as a functional starter culture for control of L. Inonocytogenes without compromising the unique quality of Nham. (C) 2011 Elsevier Ltd. All rights reserved.
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