期刊
FOOD CONTROL
卷 20, 期 4, 页码 430-432出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2008.07.007
关键词
Real-time PCR; Listeria monocytogenes; Raw whole milk
资金
- Public Health Department of Boyaca
- Molecular Diagnostics Division (Roche, Colombia)
- Biological Sciences Faculty Universidad de los Andes
The aim of Our work was to detect Listeria monocytogenes in raw whole milk from five municipalities of Boyaca-Colombia using real-time PCR. A PCR hybridization probe format was used to analyze the presence of L. monocytogenes, using specific primers to amplify a 149 bp fragment from the metalloprotease gene (mpl). In this study on a total number of 81 samples, 21 gave positive results for L. monocytogenes by real-time PCR and 13 samples were positive by conventional method. These results indicate a high presence of this pathogen in this area of the country and that this method is considerably faster than current standard methods. (c) 2008 Elsevier Ltd. All rights reserved.
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