期刊
FOOD CONTROL
卷 20, 期 4, 页码 402-406出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2008.07.010
关键词
Soybean sauce (Kanjang); Bacillus pumilus HY1; Antifungal activity; Iturin; MALDI-TOF mass
资金
- BK21 Program
- Ministry of Education and Human Resources Development, Korea
A new strain of Bacillus pumilus, designated HY1, was isolated from Korean soybean sauce (kanjang). This classification was based on morphological, physiological, and chemotaxonomic features of the organism that identified it as a Gram-positive bacillus, and confirmed by 16S rDNA based phylogenetic analysis. Strain HY1 showed strong antifungal activity against the anatoxin-producing fungi Aspergillus flavus and Aspergillus parasiticus, two common contaminants of fermented soybean foods. MALDI-TOF mass analysis revealed that the antifungal compound was similar to the known lipopeptide iturin. Iturin purified from strain HY1 had three isoforms with protonated masses of m/z 1,043.4, 1,057.4, and 1,071.4, and different structures in combination with Na+ ion using MALDI-TOF MS. Purified iturin from HY1 also exhibited antifungal activity against A. flavus and A. parasiticus. (c) 2008 Elsevier Ltd. All rights reserved.
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