期刊
FOOD CHEMISTRY
卷 154, 期 -, 页码 211-216出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.01.013
关键词
Organic UV absorbing substances; On-line silylation; Matrix solid-phase dispersion; GC MS/MS; Fish sample analysis
资金
- National Science Council of Taiwan [NSC 100-2113-M-008-001-MY3]
A simple and effective method for the rapid determination of five salicylate and benzophenone-type UV absorbing substances in marketed fish is described. The method involves the use of matrix solid-phase dispersion (MSPD) prior to their determination by on-line silylation gas chromatography tandem mass spectrometry (GC-MS/MS). The parameters that affect the extraction efficiency were optimized using a Box-Behnken design method. The optimal extraction conditions involved dispersing 0.5 g of freeze-dried powdered fish with 1.0 g of Florisil using a mortar and pestle. This blend was then transferred to a solid-phase extraction (SPE) cartridge containing 1.0 g of octadecyl bonded silica (C18), as the clean-up co-sorbent. The target analytes were then eluted with 7 mL of acetonitrile. The extract was derivatized on-line in the GC injection-port by reaction with a trimethylsilylating (TMS) reagent. The TMS-derivatives were then identified and quantitated by GC-MS/MS. The limits of quantitation (LOQs) were less than 0.1 ng/g. (C) 2014 Elsevier Ltd. All rights reserved.
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