期刊
FOOD CHEMISTRY
卷 161, 期 -, 页码 120-126出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.03.043
关键词
Acetoin; Box-Behnken design; Desirability function; Response surface methodology; Ultrasound-assisted reverse micelles; dispersive liquid-liquid microextraction
资金
- Graduate School and Research Council of the University of Yasouj
A novel approach, ultrasound-assisted reverse micelles dispersive liquid-liquid microextraction (USA-RM-DLLME) followed by high performance liquid chromatography (HPLC) was developed for selective determination of acetoin in butter. The melted butter sample was diluted and homogenised by n-hexane and Triton X-100, respectively. Subsequently, 400 mu L of distilled water was added and the microextraction was accelerated by 4 min sonication. After 8.5 min of centrifugation, sedimented phase (surfactant-rich phase) was withdrawn by microsyringe and injected into the HPLC system for analysis. The influence of effective variables was optimised using Box-Behnken design (BBD) combined with desirability function (DF). Under optimised experimental conditions, the calibration graph was linear over the range of 0.6-200 mg L-1. The detection limit of method was 0.2 mg L-1 and coefficient of determination was 0.9992. The relative standard deviations (RSDs) were less than 5% (n = 5) while the recoveries were in the range of 93.9-107.8%. (C) 2014 Published by Elsevier Ltd.
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