A rapid immunomagnetic beads-based immunoassay for the detection of β-casein in bovine milk

An immunomagnetic beads-based enzyme-linked immunosorbent assay (IMBs-ELISA) was developed for the detection of beta-casein in bovine milk. Immunomagnetic beads (IMBs) were employed as the solid phase. The anti-beta-casein monoclonal antibody (McAb) bound to IMBs was used as capture probe and an anti-beta-casein polyclonal antibody (PcAb), labelled with horseradish peroxidase (HRP), was employed as detector probe. Three reaction and two washing steps were needed. Each reaction needed 10 min or less, which significantly shortened detection compared with classic sandwich ELISA. beta-Casein in bovine milk was detected across a linear range (2-128 mu g mL(-1)). Application results were in accordance with the Kjejdahl method, which suggests the IMBs-ELISA is rapid and reliable for the detection of beta-casein in bovine milk. (C) 2014 Elsevier Ltd. All rights reserved.