期刊
FOOD CHEMISTRY
卷 162, 期 -, 页码 172-175出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.04.063
关键词
Ultra-performance convergence chromatography (UPC2); Diode array detector; Biogenic amines; Determination; Fermented foods
资金
- Special Fund for Ago-scientific Research in the Public Interest [201303077-4]
- Scientific and Technologic Funds of Key Laboratory of Tropical Crop Products Processing [KLTCPP-201402]
A rapid ultra-performance convergence chromatography (UPC2) method for the determination of eight biogenic amines (spermine, spermidine, putrescine, cadaverine, tryptamine, phenylethylamine, histamine and tyramine) in fermented foods was developed. The amines were pre-column derivatized with dansyl chloride and separated on UPC2 system with a HSS C-18 SB column (3.0 x 100 mm, 1.8 mu m) using gradient elution with a binary system of CO2 and n-hexane:isopropanol:ammonium hydroxide (70:30:0.15, v/v/v), back pressure of 2000 psi, flow rate of 2.0 ml/min and DAD detection at 254 nm. The result showed excellent linearity (r = 0.9995-1.0000). Limits of detection (LOD) and limits of quantification (LOQ) were 21-67 ng/L and 72-224 ng/L, respectively. Relative standard deviations (RSD) for repeatability and reproducibility were 0.21-0.87% and 1.98-4.02%, respectively. Furthermore, this method was successfully applied to analysis of biogenic amines in Yulu and Sufu samples. (C) 2014 Elsevier Ltd. All rights reserved.
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