期刊
FOOD CHEMISTRY
卷 146, 期 -, 页码 270-277出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.09.051
关键词
Legumes; Seeds; Saponin; UPLC-PDA-ESI/MS; Soyasaponin beta g; Methanol extract
The present work was reported on investigation of saponin profiles in nine different legume seeds, including soybean, adzuki bean, cowpea, common bean, scarlet runner bean, lentil, chick pea, hyacinth bean, and broad bean using ultra performance liquid chromatography with photodiode array detector and electrospray ionisation/mass spectrometry (UPLC-PDA-ESI/MS) technique. A total of twenty saponins were characterised under rapid and simple conditions within 15 min by the 80% methanol extracts of all species. Their chemical structures were elucidated as soyasaponin Ab (1), soyasaponin Ba (2), soyasaponin Bb (3), soyasaponin Bc (4), soyasaponin Bd (5), soyasaponin alpha g (6), soyasaponin beta g (7), soyasaponin beta a (8), soyasaponin gamma g (9), soyasaponin gamma a (10), azukisaponin VI (11), azukisaponin IV (12), azukisaponin 11 (13), AzIl (14), AzIV (15), lablaboside E (16), lablaboside F (17), lablaboside D (18), chikusetusaponin IVa (19), and lablab saponin I(20). The individual and total saponin compositions exhibited remarkable differences in all legume seeds. In particular, soyasaponin beta a (8) was detected the predominant composition in soybean, cowpea, and lentil with various concentrations. Interestingly, soybean, adzuki bean, common bean, and scarlet runner bean had high saponin contents, while chick pea and broad bean showed low contents. (C) 2013 Elsevier Ltd. All rights reserved.
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