Evaluation of matrix solid-phase dispersion (MSPD) extraction for multi-fenicols determination in shrimp and fish by liquid chromatography-electrospray ionisation tandem mass spectrometry

A quantitative LC-MS/MS method was developed for the determination of chloramphenicol (CAP), thiamphenicol (TAP), florfenicol (FF) and florfenicol amine (FFA) in shrimp and fish. This rapid simple and effective extraction method was based on matrix solid-phase dispersion (MSPD). The best results were obtained using C-18 as dispersant sorbent. The correlation coefficient (r) with each matrix-matched calibration curve is higher than 0.999 at the range of 0.05-0.8 mu g/kg for CAP and FF, 0.1-1.6 mu g/kg for FFA and TAP. CC alpha and CC beta of the fenicols upon the method were ranged from 0.01 to 0.09 mu g/kg and 0.04 to 0.25 mu g/kg respectively. In the fortified levels recoveries of the four compounds ranged from 83.8% to 98.8% with RSDs lower than 13.7%. The proposed method has been applied successfully to the analysis of CAP, TAP, FF and FFA in shrimp and fish samples, which demonstrates that this method is fast, sensitive, reliable and environmental friendly. (C) 2013 Elsevier Ltd. All rights reserved.