期刊
FOOD CHEMISTRY
卷 145, 期 -, 页码 522-529出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.08.065
关键词
Eugenia uniflora; Pressurised fluid extraction; High performance liquid chromatography; Centrifugal partition chromatography; High resolution mass spectrometry
资金
- FAPESP (State of Sao Paulo Research Foundation, Brazil) Project [2008/00148-0]
Brazilian cherry seeds are a waste product from juice and frozen pulp production and, the seeds composition was investigated to valorize this by-product. Compounds separation was performed with ethanol by pressurised fluid extraction (PFE). Here we determine the effect of temperature (7), static time (ST), number of cycles (C), and flush volume (VF) on the yield, composition and total phenolic content (TPC) of the seed extracts. T, ST and their interaction positively influenced yield and TPC. Extracts were fractionated by high performance liquid chromatography (HPLC) and centrifugal partition chromatography (CPC). The collected fractions characterizations were made by electrospray ionisation mass spectrometry (ESI/MS) and high resolution mass spectrometry (HRMS) indicated the presence of ellagic acid pentoside and deoxyhexose, quercitrin and kaempferol pentoside. All of these compounds have antioxidant properties and normally are found in plant extracts. These results confirm that Brazilian cherry seed extract is a potentially valuable source of antioxidants. (C) 2013 Elsevier Ltd. All rights reserved.
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