期刊
FOOD CHEMISTRY
卷 145, 期 -, 页码 404-408出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.08.068
关键词
Honey; Phenolic acids; Flavonoids; SPE; HPLC-ESI-MS/MS; SRM
资金
- European Social Fund
- state budget
- Polish Ministry of Science and Higher Education [3270/B/P01/2011140]
- European Regional Development Found in the Sectorial Operational Programme Improvement of the competitiveness of Enterprises [WPK_1/1.4.3./1/2004/72/721165/2005/U]
A simple, fast and specific high performance liquid chromatography separation with an electro-spray ionisation tandem mass spectrometry detection in a negative single reaction ion monitoring scan mode was developed and used for the characterization of Polish honeys according to the content of phenolic acids, including caffeic, chlorogenic, p-coumaric, ferulic, homogentisic, p-hydroxybenzoic and vanillic acids, and flavonoids, i.e., apigenin, genistein, hesperetin, kaempferol, luteolin, rhamnetin, rutin, tricetin and quercetin. Target compounds were isolated and pre-concentrated from the honey matrix by means of the solid phase extraction using Strata X (500 mg) cartridges. Analysed honeys did not contain tricetin and genistein. Hesperetin was determined for the first time in heather and linden honeys while rutin in rape honey. (C) 2013 Elsevier Ltd. All rights reserved.
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