期刊
FOOD CHEMISTRY
卷 145, 期 -, 页码 542-548出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.08.092
关键词
Citrus grandis Tomentosa; Health food; Traditional Chinese medicine; Column chromatographic extraction; Gradient elution; Volatiles; Flavonoids; Polysaccharides
资金
- Science and Technology Programs of Guangdong Province [2011B020310008]
- Natural Science Funds of Guangdong Province [10151063101000002, S201101000368]
Citrus grandis Tomentosa is widely used in traditional Chinese medicine and health foods. Its functional components include volatiles, flavonoids and polysaccharides which cannot be effectively extracted through traditional methods. A column chromatographic extraction with gradient elution was developed for one-step extraction of all bioactive substances from C. grandis. Dried material was loaded into a column with petroleum ether: ethanol (8:2, PE) and sequentially eluted with 2-fold PE, 3-fold ethanol: water (6:4) and 8-fold water. The elutes was separated into an ether fraction containing volatiles and an ethanol-water fraction containing flavonoids and polysaccharides. The later was separated into flavonoids and polysaccharides by 80% ethanol precipitation of polysaccharides. Through this procedure, volatiles, flavonoids and polysaccharides in C. grandis were simultaneously extracted at 98% extraction rates and simply separated at higher than 95% recovery rates. The method provides a simple and high-efficient extraction and separation of wide range bioactive substances. (C) 2013 Elsevier Ltd. All rights reserved.
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