期刊
FOOD CHEMISTRY
卷 141, 期 3, 页码 2682-2690出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.04.064
关键词
ACE inhibitory peptides; Soybean; QSAR model
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
The importance of soy products in reducing the risk of cardiovascular disease is well documented. Our previous computation study has indicated the presence of several potent ACE inhibitory peptides within soybean proteins which needs to be identified. The aim of the study was to identify ACE inhibitory peptides from soy proteins using LC-MS/MS coupled with quantitative structure-activity relationship (QSAR) model. Soybean protein hydrolysate digested by thermolysin showed an IC50 value of 53.6 mu g/mL, decreased slightly to 51.8 mu g/mL after adding pepsin, and increased to 115.6 mu g/mL after adding trypsin. A total of 34 peptides were characterised from LC-MS/MS. Five novel tripeptides, IVF, LLF, LNF, LSW and LEF, with predicted IC50 values lower than 10 mu M were synthesized and validated. The results showed that soybean is an excellent source of ACE inhibitory peptides. (C) 2013 Elsevier Ltd. All rights reserved.
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