期刊
FOOD CHEMISTRY
卷 136, 期 2, 页码 938-946出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.09.004
关键词
Citrus juice; Endogenous metabolites; Metabolomics; Steroids; Urine
资金
- CICYT [AGL2011-23690]
- CONSOLIDER-INGENIO 'Fun-C-Food' [CSD007-0063]
- Spanish Ministry of Economy and Competitivity [CSIC 201170E041]
- predoctoralthe CSIC-JAE fellowship
Citrus juice intake has been highlighted because of its health-promoting effects. LC-MS based metabolomics approaches are applied to obtain a better knowledge on changes in the concentration of metabolites due to its dietary intake and allow a better understanding of involved metabolic pathways. Eight volunteers daily consumed 400 mL of juice for four consecutive days and urine samples were collected before intake and 24 h after each citrus juice intake. Urine samples were analysed by nanoHPLC-q-TOF, followed by principal component analysis (PCA) and Student's t-test (p < 0.05). PCA showed a separation between two groups (before and after citrus juice consumption). This approach allowed the identification of four endocrine compounds (tetrahydroaldosterone-3-glucuronide, cortolone-3-glucuronide, testosterone-glucuronide and 17-hydroxyprogesterone), which belonged to the steroid biosynthesis pathway as significant metabolites upregulated by citrus juice intake. Additionally, these results confirmed the importance of using the non-targeted metabolomics technique to identify new endogenous metabolites, up- or down-regulated as a consequence of food intake. (C) 2012 Elsevier Ltd. All rights reserved.
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