期刊
FOOD CHEMISTRY
卷 138, 期 4, 页码 2140-2145出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.12.026
关键词
Theaflavin; Peptide transport; PEPT1; AMPK
资金
- Ministry of Education, Science, Sports and Culture of Japan [22248014]
- Grants-in-Aid for Scientific Research [22248014] Funding Source: KAKEN
In the small intestine, peptide transporter 1 (PEPT1) plays a role in the transport of di- and tripeptides. In this study, we investigated whether theaflavins (TFs) affect the absorption of small peptides in human intestinal Caco-2 cells, since TFs do not penetrate through the cells and might be involved in intestinal transport systems. In transport experiments, the transport of glycyl-sarcosine (Gly-Sar, a model molecule for PEFT1 transport) and other dipeptides (Val-Tyr and Ile-Phe) were significantly reduced (P < 0.05) in TFs-pretreated cells. In TF 3 '-0-gallate-pretreated cells, Western blot analysis revealed attenuated expression of PEPT1 transporter and Gly-Sar transport was completely ameliorated by 10 mu m Compound C, an AMP-activated protein kinase (AMPK) inhibitor. In conclusion, the present study demonstrated that TFs inhibit peptide transport across Caco-2 cell monolayers, probably through suppression of AMPK-mediated PEPT1 expression, which should be considered a new bioactivity of TFs in black tea. (C) 2012 Elsevier Ltd. All rights reserved.
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