期刊
FOOD CHEMISTRY
卷 141, 期 4, 页码 3977-3983出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.06.077
关键词
RRLC-ESI-MSn; Citri Reticulatae Pericarpium; Hierarchical cluster analysis
资金
- Natural Science Foundation of China [81202898]
- Research Fund for the Natural Science Foundation of Guangdong Province (Initiation project for PHD) [S2012040007602]
A rapid resolution liquid chromatography/electrospray ionisation tandem mass spectrometry (RRLC-ESI-MSn) method has been firstly developed and validated for simultaneous determination of six bioactive flavonoids in Citri Reticulatae Pericarpium (CRP). The antiproliferative activities of the six flavonoids in CRP, namely naringin, hesperidin, nobiletin, 3,5,6,7,8,3',4'-heptamethoxyflavone, tangeretin and 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone, were evaluated and compared by Cell Counting Kit-8 Assay. Quantification was carried out on an Agilent triple quadrupole LC-MS system using multiple reaction monitoring mode. The established method was successfully applied for determination of the six flavonoids in samples collected from different regions in China. Compared with the reported analytical methods, the RALC-ESI-MSn method is powerful in quantitative analysis of multi-component in terms of time savings and sensitivity. Hierarchical cluster analysis (HCA) was also performed to differentiate and classify the samples based on the contents of the six characteristic flavonoids. The HCA results indicated that Citrus reticulata 'Chachi' samples could be easily distinguished from other CRP samples. The developed RRLC-ESI-MSn method combined with HCA might be utilised as a quality control method for CRP. (C) 2013 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据