期刊
FOOD CHEMISTRY
卷 138, 期 4, 页码 2122-2129出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.11.099
关键词
Fructus sophorae; Flavonoids; Central composite design; The response surface method; Reflux extraction
资金
- Natural Science Foundation of Jiang Su province [BK2010242]
- Suzhou Social Development Projects [SS201124]
Response surface methodology (RSM) based on a central composite design (CCD) was applied to optimise the extraction conditions for flavonoids from fructus sophorae with advantages in terms of resisting flavonoids during the whole process and maximising of extraction yield. Three aglycon forms of the flavonoids, namely, quercetin, kaempferol and isorhamnetin were quantified by high-performance liquid chromatography with ultraviolet detection (HPLC-UV) to estimate extraction yield. The combined effects of independent variables were studied and the optimal extraction conditions were obtained as ethanol concentration, 74.47%; solid-liquid ratio, 17.99 ml/g; temperature, 89.13 degrees C; and extraction time, 2.10 h. The reliability of the method was confirmed by recovery experiments, performed under optimal conditions. Recoveries indicated that flavonoids resisted the extraction conditions. The experimental extraction yield under optimal conditions was found to be 10.459%, which was well matched with the predicted values of 10.461%. (C) 2012 Elsevier Ltd. All rights reserved.
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