期刊
FOOD CHEMISTRY
卷 137, 期 1-4, 页码 18-24出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.09.083
关键词
Ligninolytic enzymes; Polyacrylamide gel; Enzyme encapsulation; Juice clarification
资金
- Natural Sciences and Engineering Research Council of Canada (Canada Research Chair) [355254]
- FQRNT [ENC 125216]
- MAPAQ [809051]
- Initiative Inde (MELS, Quebec)
The thermal stability, physico-chemical properties and effect on juice clarification of hydrogel formulations of ligninolytic enzymes from Phanerochaete chrysosporium were evaluated. The results showed that enzyme entrapment increase significantly (P < 0.05) the thermal stability of enzymes at 4 and 75 C. At 75 degrees C, maximum activity decreased to non detectable values of 7.9% for free laccase, manganese peroxidase (MnP), lignin peroxidase (LiP), respectively; to 94%, 97%, 93% for laccase, MnP and LiP entrapped into Polyacrylamide/pectin, 94%, 98%, 88% for laccase, MnP and LiP encapsulated respectively into polyacrylamide/ gelatine and to 87%, 91%, 87% for laccase, MnP and LiP entrapped, respectively into polyacrylamide/carboxymethylcellulose (CMC). When particle size and viscosity of the formulation increased, enzyme stability increased. The polyphenolic reduction and clarity amelioration in mixed juice of berry and pomegranate was more significant (p > 0.05) using encapsulated enzymes treatment than free enzymes. This suggested that enzymatic treatment was efficient for the juice clarification. (C) 2012 Elsevier Ltd. All rights reserved.
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