期刊
FOOD CHEMISTRY
卷 138, 期 1, 页码 574-580出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.10.143
关键词
Flow injection analysis (FIA); Response surface methodology; Antioxidant activity; Tea
资金
- CAPES
This study proposes a FRAP assay adapted to FIA system with a merging zones configuration. The FIA system conditions were optimised with the response surface methodology using the central composite rotatable design. The optimisation parameters studied were: the carrier flow rate, the lengths of the sample and reagent loops, and reactor length. The conditions selected in accordance with the results were: carrier flow rate of 1.00 ml/min, length of the loops 18.2 cm and length of the reaction coil 210.1 cm. The detection and quantification limits were, respectively, 28.6 and 86.8 mu mol/l Fe2+ and the precision was 1.27%. The proposed method had an analytical frequency of 30 samples/h and about 95% less volume of FRAP reagent was consumed. The FRAP assay adapted to the FIA system under the optimised conditions was utilised to determine the antioxidant activity of tea samples. (C) 2012 Elsevier Ltd. All rights reserved.
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