期刊
FOOD CHEMISTRY
卷 141, 期 2, 页码 1369-1377出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.04.012
关键词
Ginsenoside Rg(3)(S); beta-Glucosidase; alpha-L-arabinofuranosidase; Biotransformation; Mass production
The ginsenoside Rg(3)(S), which is one of the exceptional components of Korean red ginseng extract, has been known to have anti-cancer, anti-metastatic, and anti-obesity effects. An enzymatic bioconversion method was developed to obtain the ginsenoside Rg(3)(S) with a high specificity, yield, and purity. Two glycoside hydrolases (BglBX10 and Abf22-3) were employed to produce Rg(3)(S) as a 100 g unit. The conversion reaction transformed ginsenoside Rc to Rd using Abf22-3, followed by Rb-1 and Rd to Rg(3)(S), using BglBX10. It was performed in a 10 Liar fermenter at pH 6.0 and 37 degrees C for 24 h, with a high concentration of 50 mg/ml of purified ginsenoside mixture obtained from ginseng roots. Finally, 144 g of Rg(3)(S) was produced from 250 g of root extract with 78 +/- 1.2% chromatographic purity. These results suggest that this enzymatic method would be useful in the preparation of ginsenoside Rg(3)(S) for the functional food and pharmaceutical industries. (C) 2013 Elsevier Ltd. All rights reserved.
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