期刊
FOOD CHEMISTRY
卷 139, 期 1-4, 页码 702-709出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.01.055
关键词
Sea cucumber; Fucoidan; Oligosaccharide; Fucoidanase; Mass spectrum; LC-MS
资金
- National Natural Science Foundation of China [31101302]
- Specialized Research Fund for the Doctoral Program of Higher Education [20110132120015]
- National Marine Public Welfare Scientific Research Project of China [201105029]
- Program for Changjiang Scholars and Innovative Research Team in University
Sea cucumber fucoidan is a major bioactive component of sea cucumber. Sea cucumber is widely consumed in East Asian countries as healthy food. Employing the degrading enzyme from the marine bacterium strain Flavobacteriaceae CZ1127, sea cucumber (Acaudina molpadioides) fucoidan oligosaccharides were prepared by enzymatic hydrolysis. The oligosaccharide profile of the hydrolysate was determined by liquid chromatography coupled with mass spectrometry (LC-MS). With the assistance of LC-MS, four major oligosaccharides in the hydrolysate were purified. By using tandem mass spectrometry and nuclear magnetic resonance, delicate structures of the oligosaccharides were verified as alpha-L-Fucp-1 -> 3-alpha-L-Fucp(2,4OSO(3)(2-))-1 -> 3-alpha-L-Fucp, alpha-L-Fucp-1 -> 3-alpha-L-Fucp(2,4OSO(3)(2-))-1 -> 3-alpha-L-Fucp-1 -> 3-alpha-L-Fucp, alpha-L-Fucp-1 -> 3-alpha-L-Fucp(2,4OSO(3)(2-))-1 -> 3-alpha-L-Fucp-1 -> 3-alpha-L-Fucp-1 -> 3-alpha-L-Fucp-1 -> 3-alpha-L-Fucp(2,4OSO(3)(2-))-1 -> 3-alpha-L-Fucp and alpha-L-Fucp-1 -> 3-alpha-L-Fucp(2,4OSO(3)(2-))-1 -> 3-alpha-L-Fucp-1 -> 3-alpha-L-Fucp-1 -> 3-alpha-L-Fucp-1 -> 3-alpha-L-Fucp(2,4OSO(3)(2)-)-1 -> 3-alpha-L-Fucp-1 -> 3-alpha-L-Fucp. (C) 2013 Elsevier Ltd. All rights reserved.
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