期刊
FOOD CHEMISTRY
卷 141, 期 1, 页码 542-547出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.03.013
关键词
Chirality; Enantiomers; Mentha essential oils; On-line coupling RPLC-GC-MS; Stereodifferentiation
资金
- Spanish Science and Technology Commission [CTQ2006-01687, CTQ2009-09208]
- Ministry of Science and Innovation (MICINN)
A rapid and fully automated screening of chiral compounds in essential oils, aimed to the selection of natural sources of pure enantiomers of limonene and carvone, is performed by using on-line coupled reversed phase liquid chromatography with gas chromatography and mass spectrometry (RPLC-GC-MS). Essential oils obtained from Mentha spicata and Mentha piperita were analysed by direct injection into RPLC. The reported procedure includes fractionation and clean-up in RPLC, selection of the fraction to be transferred from RPLC to GC, trapping and concentration of the target compounds in the interface, thermal desorption and, finally, enantiomeric resolution and identification of chiral compounds by GC-MS. The presence of (S)-limonene and (R)-carvone as the unique enantiomeric forms existing for both compounds could be unambiguously established by transferring different volume fractions from RPLC to GC. Data obtained demonstrate high separation efficiency and well tunable selectivity in the on-line coupled RPLC-GC-MS analysis of chiral compounds. (C) 2013 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据