期刊
FOOD CHEMISTRY
卷 135, 期 3, 页码 1777-1783出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.05.111
关键词
Milk; Animal species; P-31 NMR; Phospholipids; Plasmalogens; Nutrition
资金
- Conseil Regional Provence-Alpes-Cote d'Azur (PACA)
- Application Sante des Lipides (ASL)
- French Ministries (Ministere des affaires etrangeres et Europeennes, Ministere de l'enseignement superieur et de la recherche)
- CNRS [UMR 6612]
Our objective was to identify and quantify phospholipids in milk from different species (human HM, cow CoM, camel CaM, and mare MM) using an optimised P-31 NMR spectroscopy procedure. The phospholipid fingerprints were species-specific with a broader variety of classes found in HM and MM; HM and CaM were richer in sphingomyelin (78.3 and 117.5 mu g/ml) and plasmalogens (27.3 and 24 mu g/ml), possibly important for infant development. Total phospholipid content was higher in CaM (0.503 mM) and lower in MM (0.101 mM) compared to HM (0.324 mM) or CoM (0.265 mM). Our optimised method showed good sensitivity, high resolution, and easy sample preparation with minimal loss of target molecules. It is suitable for determining the accurate composition of a large number of bioactive phospholipids with putative health benefits, including plasmalogens. and should aid in selecting appropriate ingredient sources for infant milk substitutes or fortifiers, and for functional foods dedicated to adults. (C) 2012 Elsevier Ltd. All rights reserved.
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