期刊
FOOD CHEMISTRY
卷 133, 期 4, 页码 1155-1162出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2011.11.114
关键词
Potato; Solanum tuberosum; Glycoalkaloids; MALDI-MSI; 2,5-Dihydroxybenzoic acid; Spatial distribution
资金
- Small and Medium Business Administration (SMBA), the Republic of Korea [S1065979]
- Korea Evaluation Institute of Industrial Technology (KEIT) [S1065979] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A simple and rapid analytical method for detection and spatial distribution of glycoalkaloids in potato tubers has been developed for the first time using matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI). For effective MALDI-MSI analysis, we have developed a uniform matrix coating method using 2,5-dihydroxybenzoic acid (2,5-DHB) as the preferred matrix which results in better sensitivity than 2,4,6-trihydroxyacetophenone (2,4,6-THAP) using MALDI-TOF. The relative concentrations of two major and two minor glycoalkaloids, alpha-chaconine and alpha-solanine, dehydrochaconine and dehydro-solanine, were clearly detected and distinguished in various parts of potato tuber and their relative amounts were directly compared. We also successfully showed the relative concentrations of glycoalkaloids that were accumulated by light exposure during storage using MALDI-MSI. Therefore, MALDI-MSI has been shown to be a useful technique for screening toxic and bioactive metabolites in foods and medicinal plants. (C) 2011 Elsevier Ltd. All rights reserved.
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