期刊
FOOD CHEMISTRY
卷 132, 期 1, 页码 508-517出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2011.10.017
关键词
Carotenoid; cis-Isomers; Tomato; UPLC; Antioxidant activity
资金
- Agriculture & Agri-Food Canada [205]
- University of Guelph/Ontario Ministry of Agriculture, Food and Rural Affairs (OMAFRA)
All-trans-lutein, lycopene, beta-carotene and their 22 cis-isomers in 20 tomato breeding were separated and identified by a rapid and sensitive UPLC method using a 1.7 mu m C-18 column and a new gradient mobile phase based on methanol-MTBE-water in 15 min. All-trans-carotenoids were predominant, but 9-cis, 13-cis-lutein, 5-cis, 9-cis, 13-cis, 15-cis, di-cis-lycopene, 9-cis, 13-cis, 15-cis and di-cis-beta-carotene were also found. The cis-isomers were identified using absorption around 330 nm and the Q-ratio. The total antioxidant activities as evaluated by PCL and DPPH assays were found to correlate well with the total carotenoid content, but not with the individual carotenoid or its different isomers. This paper provides an efficient analytical method for obtaining a complete picture of carotenoids in tomatoes. It can be a valuable tool for plant breeders, food processors and researchers in developing designer tomatoes and tomato-products with unique carotenoid compositions, and functional properties. (C) 2011 Elsevier Ltd. All rights reserved.
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