期刊
FOOD CHEMISTRY
卷 135, 期 3, 页码 1471-1478出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.06.004
关键词
Citrus changshanensis; Naringin; Neohesperidin; Purification; Glucose consumption; AMPK
资金
- Special Scientific Research Fund of Agricultural Public Welfare Profession of China [200903044]
- Ministry of Science & Technology of China [2012BAD33B08]
- Program for Key Innovative Research Team of Zhejiang Province [2010R50032-5]
- 111 project
Huyou (Citrus changshanensis) is rich in naringin and neohesperidin, which are natural flavanone glycosides with a range of biological activities. Among the different fruit parts, i.e. flavedo, albedo, segment membrane (SM), and juice sacs (JS), albedo showed the highest contents of both compounds, with 27.00 and 19.09 mg/g DW for naringin and neohesperidin, respectively. Efficient simultaneous purification of naringin and neohesperidin from Huyou albedo was established by the combination of macroporous D101 resin chromatography and high-speed counter-current chromatography (HSCCC). Purified naringin and neohesperidin were identified by both HPLC and LC-MS, and their effects on glucose consumption were investigated in HepG2 cells. Cells treated with naringin and neohesperidin showed increased consumption of glucose, and this was associated with increased phosphorylation of AMP-activated protein kinase (AMPK). Therefore, naringin and neohesperidin from Huyou may act as potential hypoglycaemic agents through regulation of glucose metabolism. (C) 2012 Elsevier Ltd. All rights reserved.
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