期刊
FOOD CHEMISTRY
卷 135, 期 3, 页码 1419-1424出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.05.120
关键词
Limoniastrum guyonianum; Centrifugal partition chromatography; Antioxidant activities; Fractions; Flavonoids
资金
- Tunisian Ministry of Higher Education and Scientific Research [LR10CBBC02]
- Tunisian-French Comite Mixte de Cooperation Universitaire (CMCU) [08G0917]
- SATREPES Project
The antioxidant capacities of the crude extract, aqueous and ethyl acetate partitions of Limoniastrum guyonianum Boiss. (Zita) were investigated in this study. The ethyl acetate phase exhibited a significant antioxidant activity as judged by total antioxidant activity, DPPH test and reducing power. Fractionation of this extract by centrifugal partition chromatography (CPC) using quaternary Arizona solvent systems composed of n-heptane/ethyl acetate/methanol/water led to ten fractions. The antioxidant capacities of these fractions were assessed using the same previous tests. Fraction 8 showed the highest antioxidant capacity (1291.1 mg GAE/g DR), the power ability to quench DPPH radical (IC50 = 2 mu g/ml) and to reduce Fe3+ (EC50 = 65 mu g/ml). From this fraction, three powerful flavonoids were isolated (1-3): gallocatechin (1), epigallocatechin (2) and epigallocatechin-3-O-gallate (3). These findings suggest that the antioxidative property of L. guynianum is may be related to the presence of these flavonoids, which can be used in various industrial fields. (C) 2012 Elsevier Ltd. All rights reserved.
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