期刊
FOOD CHEMISTRY
卷 134, 期 2, 页码 894-904出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.02.201
关键词
Bauhinia forficata Link; B. forficata Link subsp pruinosa (Vogel); Fortunato & Wunderlin; Flavonoids; Authenticity; Bioactivity
资金
- Fundacao para a Ciencia e a Tecnologia (FCT) [PEst-C/EQB/LA0006/2011]
- Consolider Ingenio [CSD2007-00063 FUN-C-FOOD]
- Grupo de excelencia de la region de Murcia [04486/GERM/06]
HPLC-DAD-ESI/MSn was used to ascertain the authenticity of two certified and two commercial Bauhinia forficata Link samples. Different flavonoids profiles were obtained, involving 39 compounds. Just kaempferol-3-O-(2-rhamnosyl)rutinoside was found in all analysed samples. Five compounds were common to the certified samples of B. forficata Link and B. forficata Link subsp. pruinosa (Vogel) Fortunato & Wunderlin, being kaempferol derivatives the most representative ones. The phenolic composition of B. forficata Link subsp. pruinosa (Vogel) Fortunato & Wunderlin is described herein for the first time, accounting for eight compounds, while 10 new compounds were identified in B. forficata Link. Commercial B. forficata Link showed higher contents of quercetin derivatives, in addition to the presence of myricetin derivatives and flavonoids-(galloyl)glycosides, for which the MS fragmentation pattern is reported for the first time. B. forficata Link and the two commercial samples were able to inhibit alpha-glucosidase, with EC50 values lower than that found for acarbose. Mild effects on cholinesterases were observed with the certified samples, while commercial ones were more effective. The same behaviour was observed concerning the scavenging of DPPH, nitric oxide and superoxide radicals. The presence of high contents of quercetin derivatives in commercial samples seems to directly influence their biological properties. The differences between phenolic profiles and their relation with the authenticity of commercial samples are discussed. (C) 2012 Elsevier Ltd. All rights reserved.
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