期刊
FOOD CHEMISTRY
卷 134, 期 2, 页码 1137-1140出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.02.205
关键词
Brown seaweed extract; tert-Butyl hydroperoxide; Hydrogen peroxide; 1,10-Phenanthroline; Deferoxamine mesylate; BAPTA-AM; DNA; Comet assay
资金
- Marine Institute [MFFRI/07/01]
- Department of Agriculture, Food and the Marine
The ability of brown seaweed extracts, Ascophyllum nodosum, Laminaria hyperborea, Pelvetia canaliculata, Fucus vesiculosus and Fucus serratus to protect against tert-butyl hydroperoxide (tert-BOOH) induced stress in Caco-2 cells was investigated. Oxidative stress was determined by measuring alteration in the enzymatic activity of catalase (CAT) and superoxide dismutases (SOD) and cellular levels of glutathione (GSH). L. hyperborea, P. canaliculata and F. serratus significantly protected against tert-BOOH induced SOD reduction but did not protect against the reduction in CAT activity or the increased cellular levels of GSH. The ability of F. serratus and F. vesiculosus to protect against H2O2 and tert-BOOH induced DNA damage was also assessed. The DNA protective effects of the two seaweed extracts was compared to those of three metal chelators; deferoxamine mesylate (DFO), 1,10-phenanthroline (o-phen) and 1,2-Bis(2-aminophenoxy)ethane-N.N.N',N'-tetraacetic acid tetrakis (BAPTA-AM). F. serratus and F. vesiculosus significantly protected (P < 0.05) against H2O2 (50 mu M) induced DNA damage but not tert-BOOH induced damage. (C) 2012 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据