期刊
FOOD CHEMISTRY
卷 129, 期 4, 页码 1874-1881出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2011.05.137
关键词
Amazon oils; Carotenes; Tocopherols; Tocotrienols; Validation
资金
- FAPESP [2008/56258-8, 2010/16634-0]
- CNPq [306250/2007-1, 301999/2010-4, 140283/2009-9]
- CAPES [0099-11-2]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [10/16634-0] Funding Source: FAPESP
A normal-phase HPLC method for analysis of carotenes, tocopherols and tocotrienols has been developed and validated. In this work we presented a modification to the official AOCS method for analysis of tocols which allowed simultaneous quantification of the three groups of compounds, including carotenes. Analytes were separated using a gradient mobile phase (hexane and isopropanol) and with a gradient flow rate (1-2 mL min(-1)). The column effluent was monitored by Photo Diode Array detector (PDA) set at 292 nm (tocols) and 455 nm (beta-carotene) and by fluorescence detector set at an excitation wavelength of 290 and 330 nm emission. Inter- and intra-run accuracies and precision of the analytical method were better than +/- 15%. The lower limit of quantification was 5.0 mg L-1 for the tocols and 0.1 mg L-1 for carotenes. The method has been applied for the quantification of these compounds in Amazon oils. (C) 2011 Elsevier Ltd. All rights reserved.
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