期刊
FOOD CHEMISTRY
卷 126, 期 3, 页码 1064-1070出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.11.127
关键词
Brown seaweed; Methanolic extract; Total Phenol Content; FRAP; DPPH; beta-Carotene bleaching; CAT; SOD; GSH; Comet assay
资金
- Strategy for Science, Technology and Innovation (SSTI)
- Food Institutional Research Measure (FIRM)
The antioxidant potential of methanolic extracts of brown seaweeds was assessed by Total Phenol Content, Ferric Reducing Antioxidant Power (FRAP), beta-carotene bleaching and the DPPH. scavenging assays. Ascophyllum nodosum, Pelvetia canaliculata, and Fucus serratus contained the highest phenol concentrations while Fucus vesiculosus and F. serratus exhibited the highest FRAP activities. F. vesiculosus and A. nodosum were the most effective extracts at scavenging DPPH radicals and preventing beta-carotene bleaching. The antioxidant activity of the seaweed extracts was also evaluated in Caco-2 cells. All extracts significantly (P < 0.05) increased glutathione (GSH) content of cells after 24 h. Caco-2 cells were also pre-treated with seaweed extract for 24 h followed by exposure to hydrogen peroxide (H2O2). Antioxidant enzyme activity (catalase (CAT) and superoxide dismutase (SOD)) was assessed and DNA damage was measured using the comet assay. P. canaliculata was the most effective at preventing H2O2-mediated SOD depletion in Caco-2 cells while F. serratus exhibited the best DNA protective effects. (C) 2010 Elsevier Ltd. All rights reserved.
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