Novel peptides derived from egg white protein inhibiting alpha-glucosidase

The aim of this study was to search and identify potential anti-diabetic peptides with alpha-glucosidase and alpha-amylase inhibitory activities. After the Alcalase hydrolysis, egg white protein hydrolysates were purified and identified by LC-MS-MS. Eight identified peptides were further synthesized by the Fmoc solid-phase synthesis. The anti-diabetic activities of these synthetic peptides were assessed using enzymatic inhibitory assays against the alpha-glucosidase and alpha-amylase. Among the eight peptides, peptide RVPSLM was discovered as a potential alpha-glucosidase inhibitor with an IC(50) value at 23.07 mu mol L(-1). However, it did not exhibit a visible or detectable inhibitory efficiency on the alpha-amylase. These studies indicate the potential of using egg white protein hydrolysates as a functional food product with the anti-diabetic activity. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.