期刊
FOOD CHEMISTRY
卷 124, 期 2, 页码 603-608出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.06.082
关键词
Puerarin glucoside; HPLC-UV-MS; Antioxidative property; Maltogenic amylase; Transglycosylation; Archaea
资金
- National Natural Science Foundation of China
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
- Scientific and Technological Bureau of Changchun City [09GH06]
Puerarin is helpful for the prevention and treatment of various cardiovascular diseases. However, its insolubility often limits its uses. Transglycosylation reaction between 1% puerarin and 10% beta-CD was catalysed by maltogenic amylase from archaeon Thermofilum pendens (TfMA) at 0.2 U/mg beta-CD, 90 degrees C and pH 5.5, for 1 h in order to obtain puerarin glucosides of high water solubility. Seven puerarin derivatives with different number of additional alpha-glucosyl in the reaction solution were identified by HPLC-UV-MS analysis. Although less than puerarin, the transglycosylated puerarins still fully maintained their antioxidant activities, assessed by a radical scavenging test and a reducing power assay. Therefore, the transglycosylated puerarins exhibit a greater potential to be developed into new medicines and foods. This is the first report on the flavonoids glycosylation using the transglycosylation activity of archaeal maltogenic amylase. (c) 2010 Elsevier Ltd. All rights reserved.
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