Preparation of molecular imprinted film based on chitosan/nafion/nano-silver/poly quercetin for clenbuterol sensing

A useful molecular imprinted film (MIP-CNSQ/WGE) based on chitosan/nafion/nano-silver/poly quercetin compound was prepared by a compound electrochemical method at paraffin-impregnated graphite electrode for clenbuterol (CLB) sensing, which was characterised by means of field emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy spectra (XPS), infrared spectra (IR) and electrochemical techniques to confirm the formation. The molecular imprinted film modified electrode was successfully applied to the determination of CLB with a reliable result. In optimum conditions. CLB at concentrations of 0.3-50.0 mu M could be determined with a detection limit of 0.01 mu M (3 sigma). Determination of CLB in practical samples of pork liver showed good recovery. (C) 2011 Elsevier Ltd. All rights reserved.