期刊
FOOD CHEMISTRY
卷 126, 期 1, 页码 207-212出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.10.101
关键词
Ergosterol; Macrophages; Anti-inflammation
资金
- Council of Agriculture, Executive Yuan, ROC
Ergosterol is the principal fungal sterol in edible mushrooms and yeast lees in wine. In this study, the immunoregulatory action of ergosterol was evaluated by a proteomic approach using two-dimensional gel electrophoresis to identify proteins with modified expression in LPS-challenged RAW 264.7 macrophages treated with ergosterol. By comparing the proteome signatures, alterations of multiple proteins were observed, including NF-kappa B cascade-related proteins. Rho family GTPase protein, and cytoskeleton-related proteins. The down-regulation of NF-kappa B cascade-associated proteins coincided with the inhibition of TNF-alpha production and COX-2 expression in the ergosterol-treated RAW 264.7 cells. Western blotting analysis indicated that ergosterol inhibits LPS-induced inflammation in RAW 264.7 macrophages by suppressing the NF-kappa B signalling pathway. (C) 2010 Elsevier Ltd. All rights reserved.
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