期刊
FOOD CHEMISTRY
卷 123, 期 1, 页码 157-164出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.04.023
关键词
Annurca apple; Polyphenols; Cytotoxicity; Apoptosis; HL-60 cell line; HPLC-MS; Mass spectrometry
资金
- Centro Regionale di Competenza (CRdC) Produzioni Agroalimentari in [P.O.R. 2000-2006, misura 3.16]
- Regione Campania in the framework of the Rete di Spettrometria di Massa della Campania (RESMAC)
In Annurca apples, a southern Italian variety, polyphenols were studied. The phenolic composition of Annurca apple peel was determined by HPLC coupled with electrospray negative ionisation multistage ion trap mass spectrometry (HPLC/ESI-ITMSn). In addition, the antiproliferative and pro-apoptotic activities of peel extracts enriched in polyphenols and prepared from Annurca (APE), Red Delicious (RDPE) and Golden Delicious (GPE) varieties were evaluated on an HL-60 cell line. APE exhibited the highest total phenol content among the three apple cultivars tested, as determined by Folin-Ciocalteau's procedure and HPLC-UV analysis. APE also showed higher amounts of chlorogenic acid, procyanidins, flavonols, dihydrochalcones and cyanidin-3-O-galactoside than GPE or RDPE. All three apple extracts were able to significantly decrease cell viability, from 50% to 80%, with APE appearing the most effective, while GPE was the least cytotoxic among the three samples. Similarly, APE and RDPE induced a significant increase of caspase-3 activity, whose activation represents a hallmark of apoptosis. (C) 2010 Elsevier Ltd. All rights reserved.
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