期刊
FOOD CHEMISTRY
卷 120, 期 1, 页码 327-331出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2009.10.024
关键词
Hydrogen peroxide; Fluorometry; Coumarin; Fenton reaction; Milk
资金
- National Natural Science Foundation of China [20877031, 20677019]
- Natural Science Foundation of Hubei Province (China)
A simple and highly sensitive fluorometric method is proposed for the determination of H2O2 in milk samples. In this method. non-fluorescent coumarin is oxidised to highly fluorescent 7-hydroxycoumarin by hydroxyl radicals ((OH)-O-center dot) generated in a Fenton reaction, and the oxidation product has strong fluorescence with a maximum intensity at 456 nm and can be used as a fluorescence probe for H2O2. Under the optimal conditions (2.5 x 10(-4) mol L-1 iron(II) ions, 4.0 x 10(-4) mot L-1 cournarin, solution pH 3.0, reaction time 9 min, and excitation at 346 nm), the proposed method presents wide linear responses between the fluorescence intensity and H2O2 concentration in a wide range from 2.0 x 10(-8) to 2.0 x 10(-5) mot L-1. with a detection limit (S/N = 3) of 5.0 x 10(-9) mot L-1. After possible interferences are evaluated for a series of chemical substances, the present method has been applied to the determination of hydrogen peroxide in milk with satisfactory results. (C) 2009 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据