Partial hydrolysis of soybean oil by phospholipase A1 (Lecitase Ultra)

The partial hydrolysis of soybean oil, as catalysed by phospholipase A(1) (Lecitase Ultra) in a solvent-free system, was investigated in this study. The optimal pH and temperature for the partial hydrolysis of soybean oil by phospholipase A(1) (Lecitase Ultra) were 6.8 and 40 degrees C, respectively. Phospholipase A, (Lecitase Ultra) displayed good stability over a range of pH values from 4.7 to 7.4, and at temperatures below 60 degrees C. Phospholipase A(1) (Lecitase Ultra) is postulated to possess sn-1,3-position regiospecificity towards triacylglycerols (TAGs), based on the identification of the fatty acids released from TAGs following partial hydrolysis by swine pancreatic lipase (SPL) and phospholipase A(1) (Lecitase Ultra). Alternative TAG hydrolysis routes for phospholipase A(1) (Lecitase Ultra) are postulated, and several reaction equilibrium and rate constants were determined. (C) 2010 Elsevier Ltd. All rights reserved.