期刊
FOOD CHEMISTRY
卷 123, 期 1, 页码 14-20出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.03.091
关键词
Porphyra yezoensis; Peptide; Protein; Enzymatic hydrolysis; Central composite design; Antihypertensive activity
资金
- Key Project of National 863 Plan of China [2007AA10Z321, 2007AA100404]
- Haian County Lanbo Co., Ltd.
This research was to develop a antihypertensive peptide, an efficient angiotensin converting enzyme (ACE) inhibitor (ACEI), from Porphyra yezoensis. Seven commercial enzymes were screened and then enzymatic hydrolysis conditions were optimised. The results showed that alcalase was the most effective for hydrolysis and its optimum conditions for achieving the highest antihypertensive activity of peptide were 1.5% substrate, 5% alcalase, pH 9.0, and temperature of 50 degrees C at a hydrolysis time of 60 min. The antihypertensive peptide produced under the optimum conditions had a high ACE inhibition rate of 55.0% and a low IC50 value of 1.6 g/l and remained at high stability at temperatures of 4, 25, and 37 degrees C, pH values of 2.0 and 8.0, and after pepsin and trypsin treatments. Major proteins from P. yezoensis were glutelin, albumin, and gliadin. The albumin and glutelin had higher hydrolysis rates than the gliadin, but the IC50 value of glutelin was the lowest, which indicated that the antihypertensive peptide from glutelin was more functional. (C) 2010 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据