Molecularly imprinted solid phase extraction coupled to high-performance liquid chromatography for determination of trace dichlorvos residues in vegetables

In this paper, we prepared a highly selective imprinted polymer by a room temperature ionic liquid-mediated bulk polymerization technique, using dichlorvos as the template, methacrylic acid as the functional monomers, and trimethylolpropane trimethacrylate as the cross-linker. This functionalized material was characterized by FT-IR, static and kinetic adsorption experiments, and the results showed that this imprinted sorbent exhibited good recognition and selective ability, and offered fast kinetics for the adsorption and desorption of dichlorvos. Using the prepared material as a solid phase extraction sorbent, a novel sample pre-treatment technique that can be coupled to high-performance liquid chromatography (HPLC) had been developed for determination of trace dichlorvos residues in foods. Under the selected experimental condition, the detection limit (S/N = 3) of dichlorvos was 94.8 ng L-1, and the peak area precision (RSD) for five replicate detections of 10 mu g L-1 dichlorvos was 4.41%. The blank samples of cucumber and lettuce spiked with dichlorvos at 0.005 and 0.02 mu g g(-1) levels were determined with recoveries ranging from 82.1% to 94.0%. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.