期刊
FOOD CHEMISTRY
卷 113, 期 3, 页码 808-815出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2008.08.002
关键词
Carotenoids; Fruits; Vegetables; Natural variability; HPLC; PDO/PGI; Traditional
资金
- EU [FOOD-CT-2005-513944]
- Instituto Nacional de Saude Dr. Ricardo Jorge [BIC 01/2003-II]
Carotenoids, alpha-carotene, beta-carotene, beta-cryptoxanthin, lycopene, lutein and zeaxanthin, were determined in 10 varieties of five fruit species (orange, pear, peach, apple and cherry) and five varieties of four species of vegetables (Portuguese coles, turnip greens, purslane, leaf beet and beetroot leaves) cultivated in Portugal and country traditional, the fruits being of protected designation of origin or of protected geographical indication. The determination was done by high performance liquid chromatography, using two metal free reverse phase columns, an organic mobile phase based on acetonitrile, methanol and dichloromethane and a UV-vis photodiode array detector. Identification was done by retention time and spectral analysis and quantification was based on peak area at 450 nm by external calibration. The analysed leafy vegetables are a very good source of lutein (0.52-7.2 mg/100 g) and beta-carotene (0.46-6.4 mg/100 g) while the analysed fruits have a considerably lower content of carotenoids (lutein, 0.0032-0.16 mg/100 g and beta-carotene, 0.010-0.17 mg/100 g) and a complex and variable qualitative and quantitative carotenoid composition. Most estimated relative measurement expanded uncertainties were between 0.10 and 0.31. Results indicate that the carotenoid content of the analysed items could vary with species, varieties, geographical place of production (region, site) and time of harvest, and should be addressed in the eventual production of data for food composition data bases. (C) 2008 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据