期刊
FOOD CHEMISTRY
卷 116, 期 1, 页码 114-121出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2009.02.020
关键词
Hydroxytyrosol; Biocatalysis; Toluene monooxygenases; Protein engineering; Antioxidant
Hyroxytyrosol (HTyr), an important phenol present in olives, stands out as a compound of high added value due to its exceptional antioxidant, antimicrobial and anticarcinogenic activities. This work describes the synthesis of HTyr via double hydroxylation of 2-phenylethanol (PEA) employing toluene monooxygenases (TMOs) as biocatalysts. Wild-type TMOs were initially evaluated for their ability to oxidise PEA and structurally-related substrates, providing better understanding of the factors responsible for controlling the regiospecificity. Both the length of the alkyl side chain and the presence of the hydroxyl group were found to influence the activity, possibly by interfering with the substrates' entrance into the active site. Directed evolution of toluene-4-monooxygenase of Pseudomonas mendocina KR1 led to the discovery of variant TmoA S395C with a 15-fold increase in PEA hydroxylation rate. Saturation-mutagenesis at position TmoA 1100 resulted in the finding of novel HTyr-producing variants 1100A, 1100S and 1100G. (C) 2009 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据