期刊
FOOD CHEMISTRY
卷 116, 期 3, 页码 605-610出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2008.09.037
关键词
alpha-Carotene; beta-Carotene; Harvest time; Ipomoea batatas (L) Lam.; HPLC; Sweet potato
资金
- Chun Shan Medical University, Taichung, Taiwan [CSMU-95-OM-A-093]
A HPLC method was improved to determine sweet potato carotenoids rapidly with good separation efficiency. A C30 column and a gradient solvent system consisting of methanol-acetonitrile-water (84/14/2, v/v/v, solvent A) and dichloromethane (solvent B) (a mixture of 80% A and 20% B was used initially, and then the mixing was programmed to 55% B within 15 min and kept to the end) were used for analysis. The flow rate was 1 ml/min and detection was at 450 nm. A total of 11 all-trans and cis forms of alpha- and beta-carotene in Taiwanese sweet potato (Ipomoea batatas (L.) Lam.) could be resolved within 16 min. The orange-fleshed sweet potato (Tainung 66) had higher total carotenoid content than the yellow-fleshed one (Tainung 57) at the same harvest time. The total carotenoid levels in both crops harvested at various times were in the order: October > July > April > January. (C) 2008 Elsevier Ltd. All rights reserved.
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