期刊
FOOD CHEMISTRY
卷 116, 期 1, 页码 176-182出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2009.02.028
关键词
Raman spectroscopy; Reverse micelle; Soybean proteins; 7S and 11S globulins; Main and side chain conformation
资金
- Fund of Post-doctor Science [20080440388]
- National Natural Science Foundation of China [40701173]
Raman spectroscopy was applied to investigate the main and side chain conformational changes of 7S and 11S globulins from soybean proteins using aqueous buffer and reverse micelle extraction. The 7S and 11S globulins using two extraction methods displayed typical spectral features (such as amide I, III region and the side chain conformations of particular residues) due to characteristic amino acid composition and molecular conformation. In comparison, the degree of molecular disorder increased in both globulins using reverse micelle extraction and new bands appeared. The relative amount of different structures of 7S and 11S globulins could be estimated through accurate measurement of the band intensities. Finally, the increase of the I(850)/(830) intensity ratio of Raman tyrosine doublet in I IS globulin with reverse micelle extraction suggested a change towards a more exposed state of tyrosine residues. in good agreement with the more disordered conformation taken upon reverse micelle. (C) 2009 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据