期刊
FOOD CHEMISTRY
卷 110, 期 3, 页码 769-776出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2008.03.007
关键词
water-soluble vitamins; quantification; biosensor; infant formula
Vitamin analysis is essential for quality control and development of functional foods. in this study, a biosensor-based technology developed by Biacore AB was evaluated for analysis of water-soluble vitamins B-2, B-12, folic acid, biotin, and pantothenic acid used to supplement infant formula samples. Performance parameters such as accuracy, repeatability and recovery for the five vitamins were studied. The repeatability was measured in terms of relative standard deviation (RSD) and HORRAT(r) value. The RSD for all vitamins was below 2% and the values of HORRAT(r), were 0.16, 0.10, 0.15, 0.11 and 0.22, for B2, B12, folic acid, biotin, and pantothenic acid, respectively. The recovery of vitamins ranged from 94.7% to 109.1%. Linear analyses indicated that the square of the correlation coefficient (R-2) for B-2, B-12, folic acid, biotin, and pantothenic acid were 0.993, 0.997, 0.993, 0.993 and 0.995, respectively. The results showed that the biosensor-based vitamin analysis technology is a sensitive, reliable and realistic alternative to other methods. (C) 2008 Elsevier Ltd. All rights reserved.
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