期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 65, 期 -, 页码 242-251出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2013.12.030
关键词
Licochalcone B; Bladder cancer; S phase arrest; Apoptosis
资金
- Science and Technology Major Project of Gansu Province, China [1203FKDA032]
- Traditional Chinese Medicine Scientific Research Projects of Gansu Province, China [GZK-2009-5]
- National Natural Science Foundation of China [81260338]
- Xinjiang Production and Construction Corps Funds for Distinguished Young Scientists [2011CD006]
- International Cooperation Projects [2012BC001]
To examine the mechanisms by which licochalcone B (LCB) inhibits the proliferation of human malignant bladder cancer cell lines (T24 and EJ) in vitro and antitumor activity in vivo in M849 (murine bladder cancer cell line) tumor model. Exposure of T24 or EJ cells to LCB significantly inhibited cell lines proliferation in a concentration-dependent and time-dependent manner, and resulted in S phase arrest in T24 or EJ cells, respectively. LCB treatment decreased the expression of cyclin A, cyclin-dependent kinase (CDK1 and CDK2) mRNA, cell division cycle 25 (Cdc25A and Cdc25B) protein. In addition, LCB treatment down-regulated Bcl-2 and survivin expression, enhanced Bax expression, activated caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP) protein. Consistently, the tumorigenicity of LCB-treated MB49 cells was limited significantly by using the colony formation assay in vitro and the MB49 tumor model performed in C57BL/6 mice in vivo. These findings provide support for the use of LCB in chemoprevention and bladder cancer therapy. (C) 2013 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据