期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 60, 期 -, 页码 83-91出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2013.07.036
关键词
Apigenin; Chemotherapeutic drugs; GSK-3 beta; Luteolin; NF-kappa B; Pancreatic cancer cells
资金
- USDA from the National Institute of Food and Agriculture to the Division of Nutritional Sciences, University of Illinois [2008-03560]
- USDA Cooperative State Research, Education and Extension Service (CSREES) [AG 2010-34505-15767]
- Division of Nutritional Sciences Vision [20/20]
The objectives were to assess the potential of dietary flavonoids apigenin (Api) and luteolin (Lut) to enhance the anti-proliferative effects of chemotherapeutic drugs on BxPC-3 human pancreatic cancer cells and to investigate the potential molecular mechanism of action. Simultaneous treatment or pretreatment (0, 6, 24 and 42 h) of flavonoids and chemotherapeutic drugs at various concentrations (0-50 mu M) were assessed using the MTS cell proliferation assay. Simultaneous treatment with either flavonoid (13,25 or 50 mu M) and chemotherapeutic drugs 5-fluorouracil (5-FU, 50 mu M) or gemcitabine (Gem, 10 mu M) for 60 h resulted in mostly less-than-additive effects (p < 0.05). Pretreatment for 24 h with 13 mu M of either Api or Lut, followed by Gem for 36 h was optimal to inhibit cell proliferation. Pretreatment of cells with 11-19 mu M of either flavonoid for 24 h resulted in 59-73% growth inhibition when followed by Gem (10 mu M, 36 h). Lut (15 mu M, 24 h) pretreatment followed by Gem (10 mu M, 36 h), significantly decreased protein expression of nuclear GSK-3 beta and NF-kappa B p65 and increased pro-apoptotic cytosolic cytochrome c. Pretreatment of BxPC-3 human pancreatic cancer cells with low concentrations of Api or Lut effectively aid in the anti-proliferative activity of chemotherapeutic drugs. (C) 2013 Elsevier Ltd. All rights reserved.
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