期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 50, 期 3-4, 页码 485-491出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2011.11.030
关键词
Ethanol; Fatty liver; Garlic oil; PPAR-alpha; SREBP-1c; CYP2E1
资金
- National Science Foundation of China [81102153]
- Independent Innovation Foundation of Shandong University, IIFSDU [2010GN045]
- Scientific and Technological Cooperation Project of Shandong Province [2008GG2NS02012]
- China Postdoctoral Science Foundation [20100481253]
- Postdoctoral Science Foundation of Shandong Province [201002020]
Garlic oil (GO) has been shown to partially attenuate ethanol-induced fatty liver, but the underlying mechanisms remain unclear. The current study was designed to evaluate the protective effects of GO against ethanol-induced steatosis in vitro and in vivo, and to explore potential mechanisms by investigating the sterol regulatory element binding protein-1c (SREBP-1c), peroxisome proliferators-activated receptor-alpha (PPAR-alpha), cytochrome P4502E1 (CYP2E1), and etc. In the in vitro study, human normal cell LO2 was exposed to 100 mM ethanol in the presence or absence of GO for 24 h. We found that ethanol increased the protein levels of n-SREBP-1c and CYP2E1, but decreased the protein levels of PPAR-alpha, which was significantly attenuated by GO co-treatment. In the in vivo study, male Kun-Ming mice were pre-treated with single dose of GO (50-200 mg/kg body weight) at 2 h before ethanol (4.8 g/kg body weight) exposure. The changes of n-SREBP-1c, PPAR-alpha and CYP2E1 were paralleled well to those of in vitro study. Furthermore, GO significantly reduced the protein levels of fatty acid synthase (FAS), and suppressed ethanol-induced hepatic mitochondrial dysfunction. These results suggested that GO had the potential to ameliorate alcoholic steatosis which might be related to its modulation on SREBP-1c, PPAR-alpha, and CYP2E1. (C) 2011 Elsevier Ltd. All rights reserved.
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