期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 49, 期 1, 页码 61-69出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2010.09.032
关键词
Coptis chinensis; Berberine; Inflammatory cytokines; Toll-like receptor 4; Nuclear factor kappa B; Reactive oxygen species
资金
- Ministry of Science and Technology of the People's Republic of China [2006 BAD12B05-10]
- NSFC [30973960]
- State Key Laboratory of Animal Nutrition [2004DA125184-0810]
Coptis chinensis has been used in traditional Chinese medicine to treat inflammatory symptoms. Berberine is the main alkaloid compound of C. chinensis. This study utilized a typical lipopolysaccharide (LPS) injured model to investigate the effects of C. chinensis aqueous extract (CCAE) and berberine (major active ingredient in CCAE) in the gut-derived sepsis. In rats, pretreatment with different doses of berberine (30 or 120 mg/kg bw, i.g.; BBR30 or BBR120) or CCAE (containing 9.9% berberine; 300 mg/kg bw, i.g.; CCAE300) prior to the administration of LPS (20 mg/kg bw, i.p.) significantly suppressed the increased tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) and nitrite oxide (NO) in plasma as well as the activation of toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-kappa B) in ileum. In addition, CCAE300 and BBR30 markedly elevated the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px); significantly prevented the increased malondialdehyde (MDA), NO and villi injury in ileum compared with the negative control. Collectively. CCAE300 and BBR30 reduced the LPS-induced intestinal damage by elevating the activities of SOD and GSH-Px and by suppressing the activation of TLR4 and NF-kappa B in ileum. These results indicate that CCAE and berberine are promising agents for preventing sepsis and its complications. (c) 2010 Elsevier Ltd. All rights reserved.
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